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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Trends Microbiol. 2017 Jul 5;25(12):1016–1026. doi: 10.1016/j.tim.2017.06.003

Figure 1.

Figure 1

Methods for detecting specialized metabolites that impact biofilm formation. Phenotypic assays that look for effects on biofilm formation include observing colony morphology, biofilm reporter activity, or biofilm architecture and attachment (using confocal microscopy and crystal violet staining) in the presence of purified compound or in co-culture. Imaging mass spectrometry can be used to identify specialized metabolites in respect to their spatial distribution.