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. Author manuscript; available in PMC: 2018 Dec 15.
Published in final edited form as: Exp Cell Res. 2017 Oct 26;361(2):265–276. doi: 10.1016/j.yexcr.2017.10.026

Fig. 4.

Fig. 4

MMP activity is required for decreased cell-ECM adhesion in VANGL2 knockdown HT-1080 cells. (A) Gelatin zymography analysis of non-targeting control (NT) and VANGL2 siRNA transfected cells untreated or treated with GM6001. The 68 kDa pro-enzyme and 62 kDa mature protein are indicated. The numbers 1 and 2 on the zymogram represent results from an experiment performed in duplicate. (B,C) Quantification of cell adhesion to fibronectin (Fn) and vitronectin (Vn) by control and VANGL2 siRNA transfected cells untreated or treated with GM6001. Data are shown as percent of the NT non-drug treated control. Significant differences between untreated VANGL2 siRNA transfected cells and drug treated VANGL2 siRNA transfected cells are marked as follows: **p < 0.001; not significant (n.s.).