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. 2017 Nov 20;8:1577. doi: 10.3389/fimmu.2017.01577

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Copyright © 2017 Monguió-Tortajada, Roura, Gálvez-Montón, Franquesa, Bayes-Genis and Borràs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD73 is also induced in vivo in host monocytes in swine post-infarcted myocardium treated with porcine cATMSCs. (A–C) Infiltrating CD163⁺ monocytes (white arrows), some of them expressing CD73 (red arrows) in a cATMSC-treated animal (A), and CD163⁺ monocytes (white arrows) in a control (B) and sham (C) animals, both negative for CD73. CD73 is shown in red and CD163 in white, and nuclei are counterstained with DAPI in blue. Scale bars = 50 µm. (D,E) Histograms showing the number of CD163⁺ monocytes (D), CD73⁺ CD163⁺ monocytes (E) per optical field in all studied groups. (F) Percentage of CD73⁺ monocytes out of the CD163⁺ monocytes. Differences in immunohistochemical quantifications were compared using one-way ANOVA for multiple comparisons, with Tukey’s test for the post hoc analysis. cATMSCs, cardiac adipose tissue-derived MSCs; DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride.