Fig. 2.

Pollen developmental stages of maize inbred line B73. a Stages in relation to anther, b tassel size and c vegetative development (V) stages. Average percentages of isolated/pooled cells of V-stages of maize pollen development are shown in (c). d Isolated tetrad stages at V12 stage; bar = 10 µm. e Pooled sample at the unicellular and bicellular stage isolated at V14 stage; bar = 50 µm. (*) Indicates significant differences relative to the previous developmental stage at P < 0.001. Dots plotted represent outliers of the interquartile range of the individual measurements. Error bars represent SD (n = 5 plants for each V-stage) in at least five independent experiments