Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 20;8:1602. doi: 10.3389/fimmu.2017.01602

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Liu, Min, Wang, Wang, Ma, Dong, Zhang, Wu, Li, Zhou and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

iC3b-containing serum has negative effects on natural killer (NK) cell activity and function. Purified human NK cells from healthy donors were incubated with or without 10% of the following sera [including zymosan-treated normal serum (iC3b-S), zymosan-treated C3-depleted serum (Control-S) or iC3b-S plus 50 mM N-acetyl-d-glucosamine (NADG)] for different time periods. (A) Flow cytometric analysis of deposition of iC3b on NK cells (1 h after the incubation) using mouse anti-human iC3b (neo) antibody. A representative of three independent experiments with three cell preparations is shown. (B,C) Flow cytometric analysis of surface expression of activating [CD54, NKp46, CD69, in (B)] and inhibitory (CD158b), in (C) receptors on NK cells (24 h after the incubation). (D–F) Flow cytometric analysis of expression of NK cell functional markers (CD107a, Granzyme B, and perforin) and production of IFN-γ in NK cells that had been incubated with serum for 24 h and further stimulated with K562 tumor cells. (B–F) The results were derived from six to eight experiments using different blood donors and analyzed by paired two-tailed Student’s t-test. *P < 0.05, **P < 0.01.

iC3b-containing serum has negative effects on natural killer (NK) cell activity and function. Purified human NK cells from healthy donors were incubated with or without 10% of the following sera [including zymosan-treated normal serum (iC3b-S), zymosan-treated C3-depleted serum (Control-S) or iC3b-S plus 50 mM N-acetyl-d-glucosamine (NADG)] for different time periods. (A) Flow cytometric analysis of deposition of iC3b on NK cells (1 h after the incubation) using mouse anti-human iC3b (neo) antibody. A representative of three independent experiments with three cell preparations is shown. (B,C) Flow cytometric analysis of surface expression of activating [CD54, NKp46, CD69, in (B)] and inhibitory (CD158b), in (C) receptors on NK cells (24 h after the incubation). (D–F) Flow cytometric analysis of expression of NK cell functional markers (CD107a, Granzyme B, and perforin) and production of IFN-γ in NK cells that had been incubated with serum for 24 h and further stimulated with K562 tumor cells. (B–F) The results were derived from six to eight experiments using different blood donors and analyzed by paired two-tailed Student’s t-test. *P < 0.05, **P < 0.01.