Figure 2.

Differential inflammasome activation requirements in bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) are morphotype-dependent in Fonsecaea pedrosoi infection. IL-1β detection by ELISA in supernatants of BMDMs (A) and BMDCs (B) incubated for 24 h with medium, F. pedrosoi conidia or hyphae, and co-stimulated (or not) with LPS (500 ng/mL added 2 h before fungal infection), nigericin (20 µM during the last 40 min of incubation), or both (as a positive control). Data shown are mean ± SEM and are representative of three independent experiments. **p < 0.01; ***p < 0.001, compared to cells with only medium.