Figure 7.

NLRP3 inflammasome does not contribute to fungal clearance in a murine chromoblastomycosis model. Wild-type (WT), Nlrp3^−/− and Caspase-1/11^−/− C57BL/6 mice (n = 4) were infected in both hind footpads with 1 × 10⁶ Fonsecaea pedrosoi propagules. WT uninfected mice were used as control. Mice were euthanized at the indicated days postinfection, and the footpad tissue homogenate was assessed for both cleaved and uncleaved IL-1β (A) and interleukin-18 (B) by ELISA, and plated for colony-forming unit analysis (C). Data shown are mean ± SEM and are representative of two independent experiments. *p < 0.05; **p < 0.01, compared to WT infected.