Fig. 1.

Deletion of TRC40 has differential effects on endogenous TA protein levels. (A) The indicated tail-anchored (TA) proteins were analysed by immunoblotting extracts from HeLa M cells and ΔTRC40 cells transfected with pcDNA5 as a vector control, and ΔTRC40 cells rescued by TRC40-V5 transfection. (B) Quantification of TA protein steady-state levels from A relative to α-tubulin (α-Tub) as a cell lysate loading control. HeLa M sample quantifications from each immunoblot were analysed as the control and other conditions were calculated relative to the HeLa M sample in each biological repeat. Data shown are mean±s.d. (n=3 biological replicates) and asterisk indicates the significance level between samples shown with a horizontal line. *P≤0.05; ns, not significant (two-way ANOVA).