Skip to main content
. 2017 Nov 1;130(21):3713–3727. doi: 10.1242/jcs.206318

Fig. 3.

Fig. 3.

OXPHOS protein complexes, mtDNA, mitochondrial content and mitochondrial biogenesis signaling are upregulated in sAC KO MEFs. (A) Western blot of OXPHOS complex subunits and structural components of the mitochondria machinery in cell homogenates (n=3−6). (B) Quantification of the relative contents of OXPHOS complexes normalized to β-actin (n=3−6). (C) mtDNA content measured by qPCR of the COX1 gene normalized to that of the 18S rRNA gene (n=4). (D) Mitochondrial density expressed as the average area of mitotracker-Green-labeled mitochondria in each cell (n=3, >20 cells per experiment). (E) Western blot of AMPK and P-AMPK (Tyr 172). (F) Quantification of the P-AMPK:AMPK intensity ratio (n=4). (G) PGC-1α mRNA levels relative to β-actin (n=4). (H) NRF1 mRNA levels relative to β-actin (n=4). All data are expressed as % of wt. Data are expressed as mean±s.e.m. in indicated number of different biological replicates (*P<0.05, **P<0.01, ***P<0.001, Student's t-test). (I) PGC-1α mRNA levels relative to β-actin in cells treated with (+D) or without Dorsomorphin (n=3). Data are expressed as mean±s.e.m. in indicated number of different biological replicates (***P<0.001, ANOVA with Tukey's correction).