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. 2017 Nov 1;144(21):3894–3906. doi: 10.1242/dev.150391

Fig. 4.

Fig. 4.

Gastruloids do not express genes associated with extra-embryonic tissues and progressively activate posterior markers. Quantitative RT-PCR analysis of gastruloids at 24, 48 and 72 h AA for genes associated with the epiblast, extra-embryonic tissues or those expressed in both tissues (n=∼64 gastruloids per time-point). Gastruloids display a more differentiated phenotype over time, with little detectable expression of genes associated with the extra-embryonic tissues.