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. 2017 Nov 1;144(21):3894–3906. doi: 10.1242/dev.150391

Fig. 7.

Fig. 7.

Wnt/β-catenin signalling between 48 and 72 h AA is essential for the correct position and expression of T/Bra. (A) Examples of the morphology and expression of T/Bra::GFP gastruloids stimulated with Chi between 24 and 72 h (top, n=14), 48 and 72 h (middle, n=14), and 48 and 96 h (bottom, n=13) AA; and (B) the corresponding fluorescence and shape-descriptor quantification. Multiple poles of expression and stunted elongations are observed when Chi is applied between 24 and 72 h AA, whereas longer later stimulation (48-96 h) results in wider gastruloids and less well defined T/Bra::GFP expression, compared with the 48-72 h control (refer to Fig. S9 for further details and statistical analysis). Scale bars: 100 μm.