Figure 3.

v-PL effect on cell proliferation—cell lines. The human cell lines U937, HeLa, HaCaT, and the hamster V79 cell line were cultured in the presence of different platelet- and plasma-derived supplements and their proliferation rate was monitored by calculating the number of doublings performed at different culture times. On the contrary to the behavior of the primary cell cultures, cell lines are less responsive to the v-PL mitogenic stimulus, and the culture condition in the presence of only serum is permissive to the proliferation of the cells both in adhesion or in suspension. Number (n) of cell cultures used for the experiments is indicated on the graph for each cell type.