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. 2017 Nov 20;5:66. doi: 10.3389/fbioe.2017.00066

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Copyright © 2017 Muraglia, Nguyen, Nardini, Mogni, Coviello, Dozin, Strada, Baldelli, Formica, Cancedda and Mastrogiacomo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maintenance of the differentiation potential by cells cultured in the presence of v-PL and Pl-s. (A,B,E,F) Primary cultures of adipose-derived mesenchymal stem cells (AD-MSC) (passage 2) cultured in 10%Pl-s + 1%v-PL (A,B) or 10% FCS (E,F); (C,D,G,H) primary cultures of bone marrow-derived mesenchymal stem cells (BM-MSC) (passage 2) cultured in 10%Pl-s + 1%v-PL (C,D) or 10% FCS (G,H); (A,C,E,G) cells osteogenically induced; (B,D,F,H) not osteogenically induced control cells. (I,L) Human articular chondrocytes cultured as adherent dedifferentiated cells [(I) scale bar 200 µm] and transferred to suspension cultures [(L) scale bar 50 µm] stained with antibodies against αII collagen.

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