Table 2.
Primers | Sequence (5′ → 3′) |
---|---|
L3-F | CCGGAATTCATGGAAATTTTTGATTATATGCG |
L3-R | CCCAAGCTTTTACCGCGGAAGCCTTTTTTC |
RTL3-F | TGAAAGAACCGAAGCACG |
RTL3-R | CCGTTCTTCCGCCATCC |
L1up-F | CCAGTACTCTGCAGAATTCGCTCCTTTCATTTGGTCAG |
L1up-R | GTAAGCAAAAATTTTAGTCATATATAATCTTCCTCCCCATC |
D-F | GATGGGGAGGAAGATTATATATGACTAAAATTTTTGCTTAC |
D-R | GAAGCCCGGCCGGCACAAATGCTTAGCCAACCTTAACTGGAG |
L1down-F | CTCCAGTTAAGGTTGGCTAAGCATTTGTGCCGGCCGGGCTTC |
L1down-R | GCCGAAAATATGCACTCGAGGATCAACCGGGTCAGTGCAG |
77-F | CTGCACTGACCCGGTTGATCCTCGAGTGCATATTTTCGGC |
77-R | CTGACCAAATGAAAGGAGCGAATTCTGCAGAGTACTGG |
pL1D-L1F | CGGGGTACCAGCCTCATCGCCGGTTTCC |
pL1D-L1R | TAGGCAACAACTTTTCTCATATATAATCTTCCTCCCCATC |
pL1D-DF | GATGGGGAGGAAGATTATATATGAGAAAAGTTGTTGCCTA |
pL1D-DR | CCCAAGCTTTCATACTTTTATCTCCCACCTGCTC |
pDD-F | CGGGGTACCGCATTCGTCTGAGTGGGCC |
pDD-R | CCCAAGCTTTCATACTTTTATCTCCCACCTG |
pL1LD-pL1F | GATTGTGAAATTGAATTCGAGCTCGGTACCAGCCTCATCGCCGGTTTCCCTCGC |
pL1LD-LDR | GAAACAGCTATGACCATGATTACGCCAAGCTTTTAGCCAACCTTAACTGGAGTTTC |
pDLD-pDF | CTGATTGTGAAATTGAATTCGAGCTCGGTACCGCATTCGTCTGAGTGGGCCAAGG |
pDLD-pDR | GTAAGCAAAAATTTTAGTCATACGACAGCTTCCTTTCCATTC |
pDLD-LDF | GAATGGAAAGGAAGCTGTCGTATGACTAAAATTTTTGCTTAC |
pDLD-LDR | CTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGTTAGCCAACCTTAACTGGAG |
Restriction sites in the primer sequences are underlined