Skip to main content
. 2017 Nov 25;16:213. doi: 10.1186/s12934-017-0827-1

Table 2.

Primers used in this study

Primers Sequence (5′ → 3′)
L3-F CCGGAATTCATGGAAATTTTTGATTATATGCG
L3-R CCCAAGCTTTTACCGCGGAAGCCTTTTTTC
RTL3-F TGAAAGAACCGAAGCACG
RTL3-R CCGTTCTTCCGCCATCC
L1up-F CCAGTACTCTGCAGAATTCGCTCCTTTCATTTGGTCAG
L1up-R GTAAGCAAAAATTTTAGTCATATATAATCTTCCTCCCCATC
D-F GATGGGGAGGAAGATTATATATGACTAAAATTTTTGCTTAC
D-R GAAGCCCGGCCGGCACAAATGCTTAGCCAACCTTAACTGGAG
L1down-F CTCCAGTTAAGGTTGGCTAAGCATTTGTGCCGGCCGGGCTTC
L1down-R GCCGAAAATATGCACTCGAGGATCAACCGGGTCAGTGCAG
77-F CTGCACTGACCCGGTTGATCCTCGAGTGCATATTTTCGGC
77-R CTGACCAAATGAAAGGAGCGAATTCTGCAGAGTACTGG
pL1D-L1F CGGGGTACCAGCCTCATCGCCGGTTTCC
pL1D-L1R TAGGCAACAACTTTTCTCATATATAATCTTCCTCCCCATC
pL1D-DF GATGGGGAGGAAGATTATATATGAGAAAAGTTGTTGCCTA
pL1D-DR CCCAAGCTTTCATACTTTTATCTCCCACCTGCTC
pDD-F CGGGGTACCGCATTCGTCTGAGTGGGCC
pDD-R CCCAAGCTTTCATACTTTTATCTCCCACCTG
pL1LD-pL1F GATTGTGAAATTGAATTCGAGCTCGGTACCAGCCTCATCGCCGGTTTCCCTCGC
pL1LD-LDR GAAACAGCTATGACCATGATTACGCCAAGCTTTTAGCCAACCTTAACTGGAGTTTC
pDLD-pDF CTGATTGTGAAATTGAATTCGAGCTCGGTACCGCATTCGTCTGAGTGGGCCAAGG
pDLD-pDR GTAAGCAAAAATTTTAGTCATACGACAGCTTCCTTTCCATTC
pDLD-LDF GAATGGAAAGGAAGCTGTCGTATGACTAAAATTTTTGCTTAC
pDLD-LDR CTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGTTAGCCAACCTTAACTGGAG

Restriction sites in the primer sequences are underlined