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. 2017 Nov 25;13:354. doi: 10.1186/s12917-017-1281-3

Table 3.

Details of primers and qPCR conditions for the putative reference genes assessed in this study

Reference gene Accession number Forward primer 5′ to 3′ Reverse primer 5′ to 3′ Product length (bp) Ta (°C)
RPS5 XM_533568 TCACTGGTGAGAACCCCCT CCTGATTCACACGGCGTAG 141 62.5
RPS19 XM_533657 CCTTCCTCAAAAAGTCTGGG GTTCTCATCGTAGGGAGCAAG 95 61
HPRT AY_283372 AGCTTGCTGGTGAAAAGGAC TTATAGTCAAGGGCATATCC 114 56
HNRNPH XM_53857 CTCACTATGATCCACCACG TAGCCTCCATAACCTCCAC 151 61.2
RPL8 XM_532360 CCATGAATCCTGTGGAGC GTAGAGGGTTTGCCGATG 64 55
GAPDH NM_001003142 TGTCCCCACCCCCAATGTATC CTCCGATGCCTGCTTCACTACCTT 100 58
B2M XM_535458 TCCTCATCCTCCTCGCT TTCTCTGCTGGGTGTCG 85 61.2
SRPR XM_03184 GCTTCAGGATCTGGACTGC GTTCCCTTGGTAGCACTGG 81 61.2
GUSB NM_001003191 AGACGTTCCAAGTACCCC AGGTGTGGTGTAGAGGAGCAC 103 62

T a annealing temperature, bp base pair