Table 3.
Reference gene | Accession number | Forward primer 5′ to 3′ | Reverse primer 5′ to 3′ | Product length (bp) | Ta (°C) |
---|---|---|---|---|---|
RPS5 | XM_533568 | TCACTGGTGAGAACCCCCT | CCTGATTCACACGGCGTAG | 141 | 62.5 |
RPS19 | XM_533657 | CCTTCCTCAAAAAGTCTGGG | GTTCTCATCGTAGGGAGCAAG | 95 | 61 |
HPRT | AY_283372 | AGCTTGCTGGTGAAAAGGAC | TTATAGTCAAGGGCATATCC | 114 | 56 |
HNRNPH | XM_53857 | CTCACTATGATCCACCACG | TAGCCTCCATAACCTCCAC | 151 | 61.2 |
RPL8 | XM_532360 | CCATGAATCCTGTGGAGC | GTAGAGGGTTTGCCGATG | 64 | 55 |
GAPDH | NM_001003142 | TGTCCCCACCCCCAATGTATC | CTCCGATGCCTGCTTCACTACCTT | 100 | 58 |
B2M | XM_535458 | TCCTCATCCTCCTCGCT | TTCTCTGCTGGGTGTCG | 85 | 61.2 |
SRPR | XM_03184 | GCTTCAGGATCTGGACTGC | GTTCCCTTGGTAGCACTGG | 81 | 61.2 |
GUSB | NM_001003191 | AGACGTTCCAAGTACCCC | AGGTGTGGTGTAGAGGAGCAC | 103 | 62 |
T a annealing temperature, bp base pair