Table 4.

Reference genes ranked based on their expression stability, M, in canine osteosarcoma primary tumors and cell lines

Primary tumors (tissues)		Cell lines
Gene	M value	Gene	M value
RPS19	0.790	HNRNPH	0.420
RPS5	0.796	RPS5	0.423
HNRNPH	0.803	B2M	0.475
HPRT	0.808	RPS19	0.494
GUSB	0.816	GUSB	0.508
GAPDH	0.835	HPRT	0.510
RPL8	0.842	GAPDH	0.510
SRPR	0.921	RPL8	0.579
B2M	1.210	SRPR	0.588

The lower the M value for a gene, the more stable expression is across the samples