Fig. 6.

MiR-139 reduces protein levels of major downstream members of GHR and IGF1R signaling in BMECs. a BMECs were transfected with Lipofectamine 2000 (Control), negative control of miR-139 mimic (Mimic NC) or miR-139 mimic. Western blotting analysis showed the protein levels of p-STAT5, STAT5, IGF1R, p-AKT1, AKT1, Cyclin D1, p-mTOR, mTOR, p-p70S6K, p70S6K and β-actin at 48 h post-transfection. b BMECs were transfected with Lipofectamine 2000 (Control), negative control of miR-139 inhibitor (Inhibitor NC) or miR-139 inhibitor. Western blotting analysis showed the protein levels of p-STAT5, STAT5, IGF1R, p-AKT1, AKT1, Cyclin D1, p-mTOR, mTOR, p-p70S6K, p70S6K and β-actin at 48 h post-transfection. c Relative protein levels were quantified by analyzing scanned blots in panel a using Bandscan5.0 software. β-actin was used as a loading control. d Relative protein levels were quantified by analyzing scanned blots in panel b using Bandscan5.0 software. β-actin was used as a loading control. For c and d, results are shown as means ± SEM from three independent experiments. Statistical analysis was conducted using one-way ANOVA. “*”, statistical significance was considered at P<0.05