Table 1.
Baseline characteristics and outcomes
| Control (n = 18) | NBO2 (n = 18) | HBO2 (n = 19) | P-value | |
|---|---|---|---|---|
| Weight, gram (SD) | 296 (24) | 281 (14) | 309 (25) | < 0.0001 |
| Temp. at beginning of OP, C° (SD) | 36.2 (0.7) | 36.4 (0.9) | 36.2 (0.6) | 0.54 |
| Temp. at end of operation, C° (SD) | 36.7 (0.6) | 36.4 (0.4) | 35.4 (0.4) | 0.97 |
| Operation time, minutes (IQR) | 50 (45–58) | 47 (34–56) | 50 (40–64) | 0.37 |
| Caecum ligated, millimeters (SD) | 62.9 (6.7) | 60.8 (6.5) | 60.3 (6.8) | 0.81 |
| Microorganisma | ||||
| Positive blood culture | 4 (57) | 4 (44) | 9 (82) | 0.16 |
| Polymicrobial | 2 (29) | 3 (33) | 3 (27) | 0.77 |
| Escherichia coli | 4 (57) | 3 (33) | 6 (55) | 0.41 |
| Enterococcus faecalis | 1 (14) | 3 (33) | 2 (18) | 0.62 |
| Other | 2 (29) | 3 (33) | 4 (36) | 0.89 |
| Cytokinesb, pg/mL | ||||
| TNF-α | 57.2 (0.4–114.9) | 11.9 (0.4–118.7) | 25.7 (0.4–102.9) | 0.99 |
| IL-6 | 33.4 (2.8–70.0) | 2.8 (2.8–153.2) | 2.8 (2.8–30.5) | 0.87 |
| IL-10 | 79.4 (0.1–155.9) | 0.1 (0.1–278.8) | 64.7 (0.1–148.5) | 0.66 |
| Blood drawn sublinguallyc | ||||
| No of subjects | 7 (17) | 10(22) | 9(17) | |
| Mortality | ||||
| 24-h | 6 (33) | 8 (44) | 4 (21) | 0.13 |
| 48-h | 10 (56) | 9 (50) | 8 (42) | 0.63 |
| 72-h | 11 (61) | 9 (50) | 8 (42) | 0.63 |
CLP cecal ligation and puncture, NBO 2 normobaric oxygen, HBO 2 hyperbaric oxygen, Temp. temperature, IL interleukin, TNF tumor necrosis factor, SD standard deviation, IQR interquartile range, OP operation
Differences between the NBO and HBO groups were tested using Chi-square test/Fisher’s exact test, student’s unpaired t-test or Mann-Whitney U-test
aBlood sampling on rats alive at time = 72 h (control n = 7; NBO n = 9; HBO n = 11). Values are reported as number of subjects (%)
bBaseline cytokine concentrations taken after CLP procedure at time = 0 h. Values are reported as mean (IQR)
cNo of samples drawn sublingually. In total 26 of 117 cases, the femoral vein catheter was obstructed. Values are reported as number of subjects (%)