Fig. 2.

PTHLH promotes ICC cell growth via altering the cell cycle. a Depletion of PTHLH suppresses ICC cell growth (**p < 0.01). b PTHLH re-expression accelerates cell growth in ICC cells. Cell proliferation was examined using the CCK-8 assay in ICC cells with the stable re-expression of PTHLH (*p < 0.05, ***p < 0.001). c The distribution of cell cycle phases in RBE cells. The values are the mean ± SD (*< 0.05, **< 0.01). d The distribution of cell cycle phases in HCCC-9810 cells. Cell population sizes averaged from three independent experiments with standard deviations (*< 0.05, **< 0.01). e Protein expression of CyclinB1, p-cdc2, cell-cycle-related proteins in the G₁ phase was determined by western blot (*< 0.05, **< 0.01, ***< 0.001)