Figure 2.

Follicle-stimulating hormone (Fsh) time-course study on igfbp transcript levels in adult zebrafish testis after 1–7 days of primary organ culture. Transcript levels of igfbp1a (A), igfbp3 (B), igfbp6a (C), igfbp2a (D), and igfbp5b (E) are expressed as fold-change compared to basal conditions (absence of Fsh; represented by a dotted line), as induced by recombinant zebrafish Fsh (100 ng/mL) (bars). The duration of the ex vivo culture varied between 1 and 7 days (n = 5–7). Asterisks indicate significant differences (*P < 0.05) compared to the respective control group.