Skip to main content
. 2017 Nov 20;8:328. doi: 10.3389/fendo.2017.00328

Figure 4.

Figure 4

Effect of 25 or 100 ng/mL Igf3 on spermatogonial proliferation and proportion of area after 3 days of primary testis tissue culture. (A) Mitotic index of type Aund, type Adiff, and type B spermatogonia in the presence of 25 ng/mL (black bars) (n = 6) or 100 ng/mL Igf3 (gray bars) (n = 7). (B) Proportion of section surface area occupied by cysts containing type Aund, type Adiff, or type B spermatogonia, following exposure to 25 ng/mL (black bars) (n = 6) or 100 ng/mL Igf3 (gray bars) (n = 7). (C–E) Immunocytochemical detection of BrdU in sections of zebrafish testis incubated under basal conditions (C) or in the presence of 25 ng/mL (D) or 100 ng/mL Igf3 (E) for 3 days, showing BrdU positive (+) and negative (−) Aund, Adiff, and B spermatogonia. Bars, 25 µm. Dotted lines in A and B represent the mean values of the control groups (absence of Igf3). Asterisks indicate significant differences (*P < 0.05; **P < 0.01; ***P < 0.001) compared to the respective control group.