Table 1.
Primers used for gene expression studies.
| Target genes | Primers name | Sequence (5′–3′) | Gene information |
|---|---|---|---|
| igfbp1a | 4194 (Fw) | GAGCCCCGAGCCTAACCA | Safian et al. (23) |
| 4196 (Rv) | TCTCATAACGGGCCGACG | ||
| igfbp1b | 4199 (Fw) | GTGGAGCACCACCCTACTGAAG | Safian et al. (23) |
| 4200 (Rv) | TGCATCACCTGCTGAGCC | ||
| igfbp2a | 4206 (Fw) | GACCCTAAAGCACCACATGCTAA | Safian et al. (23) |
| 4207 (Rv) | TTGACCAGGTGCTGGAAAGG | ||
| igfbp2b | 4211 (Fw) | GCCCACCATGACCAACCA | Safian et al. (23) |
| 4213 (Rv) | GAAGTAAATGGCACGCGGTC | ||
| igfbp5a | 4226 (Fw) | CTCCCCTTCCCATCGACAA | Safian et al. (23) |
| 4227 (Rv) | CAGAAGGAAGCTGGACGGAAT | ||
| igfbp5b | 4333 (Fw) | CGCAAACATGTAAGCCCTCTAG | Safian et al. (23) |
| 4334 (Rv) | ATGGAGTTCAAATGCCGGG | ||
| igfbp6a | 4955 (Fw) | CCTCTGGTGGCGACAAATATG | Safian et al. (23) |
| 4956 (Rv) | TGCATCAACTGCCAGAACTCTAA | ||
| igfbp6b | 4928 (Fw) | TGACATCTACATCCCAAACTGTGA | Safian et al. (23) |
| 4929 (Rv) | GGAAAAAGCAGTGTCGGTCC | ||
| foxa2 | 5741 (Fw) | GTCAAAATGGAGGGACACGAAC | Potential marker for type A undifferentiated spermatogonia |
| 5743 (Rv) | CATGTTGCTGACCGAGGTGTAA | ||
| piwil2 | 2994 (Fw) | TGATACCAGCAAGAAGAGCAGATCT | Expressed in all germ cell type except type Aund spermatogonia and spermatozoa (29) |
| 2995 (Rv) | ATTTGGAAGGTCACCCTGGAGTA | ||
| dazl | 3104 (Fw) | AGTGCAGACTTTGCTAACCCTTATGTA | Expressed mainly in type B spermatogonia and primary spermatocytes (30) |
| 3105 (Rv) | GTCCACTGCTCCAAGTTGCTCT | ||
| igf1ra | 2362 (Fw) | TACATCGCTGGCAACAAGCA | Igf1 receptor a (30) |
| 2363 (Rv) | TCATTGAAACTGGTCCTTATGCAAT | ||
| igf1rb | 2595 (Fw) | GTGCTGGTCCTCTCCACACTCT | Igf1 receptor b (30) |
| 2596 (Rv) | TTACCGATGTCGTTGCCAATATC |
Fw, forward; Rv, reverse.