Table 1.
Primers and probes utilized in qPCR.
| Primer (5′–3′) Probe (5′–3′) | Primer/Probe final qPCR concentration | Portion of gene amplified | Citation |
|---|---|---|---|
| ompBRm2832F-GCGGTGGTGTTCCTAATAC | 0.2 μM | R. montanensis ompB | Petchampai et al. (2014) |
| ompBRm2937R-CCTAAGTTGTTATAGTCTGTAGTG | 0.2 μM | ||
| RmompB-HEX/CGGGGCAAAGATGCTAGCGCTTCACAGTTACCC CG/IABkFQ | 0.3 μM | ||
| RamompBEHF-CCGTTAACACCATTAACTATTAAAGCA | 0.2 μM | R. amblyommatis ompB | This paper |
| RamompBEHR-GTGCTGCGGCTTCTACATTA | 0.2 μM | ||
| RamompB-FAM/AGAGGCGCCTTTTGAGTTGTAGGATTTGC/BHQ_1 | 0.3 μM | ||
| RpompB129FJJ-CAAATGTTGCAGTTCCTCTAAATG | 0.2 μM | R. parkeri ompB | Banajee et al. (2015) |
| RpompB224RJJ-AAAACAAACCGTTAAAACTACCG | 0.2 μM | ||
| RparompB-FAM/TTTG+A+G+C+A+G+CA/IABkFQ | 0.3 μM | ||
| Rf17KD135F-ATGAATAAACAAGGKACNGGHACAC | 0.2 μM | Rickettsia 17 kDa | Jiang et al. (2004) |
| Rf17KD249R-AAGTAATGCRCCTACACCTACTC | 0.2 μM | ||
| R17Kbprobe-FAM/CGCGACCCGAATTGAGAACCAAGTAATGCGTCGCG/BHQ | 0.3 μM | ||
| ompBRr1370F-ATAACCCAAGACTCAAACTTTGGTA | 0.2 μM | R. rickettsii ompB | Jiang et al. (2005) |
| ompBRr1494R-GCAGTGTTACCGGGATTGCT | 0.2 μM | ||
| RrompB-FAM/CGCGATCTTAAAGTTCCTAATGCTATAACCCTT ACCGATCGCG/IABkFQ | 0.3 μM | ||
| AmacMIF.18F-CCAGGGCCTTCTCGATGT | 0.2 μM | A. maculatum mif | Lee et al. (2016) |
| AmacMIF.99R-CCATGCGCAATTGCAAACC | 0.2 μM | ||
| AmacMIF.63-Hex-TGTTCTCCTTTGGACTCAGGCAGC/BHQ | 0.3 μM | ||
| CRTDv321F-AGGAGAAAAGCAAGGGACTG | 0.2 μM | D. variabilis citrate synthase | Petchampai et al. (2014) |
| CRTDv452R-CAATGTTCTGCTCGTGCTTG | 0.2 μM | ||
| DvCRT_TYE665-TYE665/TGGAGAAGGGCTCGAACTTGGC/IAbRQsp + denotes the use of a locked nucleic acid (LNA). | 0.3 μM |