Table 2.

Relative expression of selected CDSs in SG of fed ticks in relation to unfed ticks by RNA-seq and RT-qPCR.

CDSs	Annotation	RNA-seq	RT-qPCR
		72 h	72 h	24 h
Acaj-81474	Glycine-rich cell wall structural protein	0.20*	0.31*	0.40*
Acaj-81475	Glycine-rich cell wall structural protein	0.24*	0.35*	0.60*
Acaj-72892	Eukaryotic translation initiation factor 4 gamma	0.06*	0.18*	0.15**
AcajSIGP-29822	Tick_cistatins_1	0.25*	0.28*	1.37
Acaj-56179	Secreted cysteine rich protein partial	3, 443.20*	454.77*	83.06*
Acaj-73764	Glycine-rich cell wall structural protein 2	164.99*	262.06**	1, 032.91*
AcajSIGP-81204	Peptidoglycan recognition protein	374.91*	263.88*	100.78**
Acaj-77950	Tick_Kunitz_135	7, 868.61*	8, 694.17**	660.38*
Acaj-74654	Glycine-rich cell wall structural protein	89.75*	95.75**	62.79**
Acaj-65746	Secreted salivary gland peptide	1, 177.56*	2, 460.95**	16.51

^* p < 0.05;

^**p < 0.001 [Statistically significant differences of expression in the SG of fed ticks in relation to the control (unfed ticks)].