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. 2017 Nov 21;8:817. doi: 10.3389/fphar.2017.00817

FIGURE 6.

FIGURE 6

Inhibition of calpain activity by oleamide in rat striatum tissue lysates. Rat striatum tissue lysates (20 μg) were incubated with μ-calpain (0.0125 U/ug) in the presence of 1 mM CaCl2 for 30 min at 37°C. OA (30, 100 μM) was co-incubated in the same conditions to assess whether the cleavage of CRMP-2 and Cdk5-p35 were mediated by calpain. Western blotting was performed to detect cleavage of CRMP-2 and Cdk5-p35.