Figure 4.

Uncertainty in dose-response curves at extrapolated drug concentrations. Current block experiments were performed for six ionic currents (see legend) with the 12 CiPA training drugs (72 drug-current combinations total with 19 excluded, see Table 3). Dose-response curves were fitted for each experiment and extrapolated above the highest experimentally tested drug concentration (C_high). Uncertainty in dose-response curves was quantified at 1×, 2×, 3×, and 10× C_high as the width of the 95% CI for the predicted percentage block, plotted as a function of the mean experimentally observed block at 1× C_high. Vertical dotted line is drawn at 60% observed mean block, denoting an approximate lower limit on the mean block that was observed at 1× C_high in experiments for which uncertainty remained low (<16%) at higher concentrations.