Table 2.
Treatments | Nodule numbers | Foliar δ15N (%0) | N concentration (%) | Nfixed (%) | Specific nodule activity (μg N mg−1 dwt nodule) | |||
---|---|---|---|---|---|---|---|---|
N+ | N− | N+ | N− | N+ | N− | |||
Bradyrhizobium elkanii H255 | 9 ± 1 a, y | 129 ± 10 a, x | −1.37 ± 0.05 a, x | −1.92 ± 0.10 a, y | 3.40 ± 0.12 a, x | 2.21 ± 0.19 a, y | 57 ± 1 a | 313 ± 13 a |
Rhizobium multihospitium-like HT221 | 4 ± 1 b, y | 45 ± 8 b, x | −1.41 ± 0.09 a, x | −2.15 ± 0.17 a, y | 3.42 ± 0.15 a, x | 1.94 ± 0.15 a, y | 51 ± 4 ab | 287 ± 26 a |
Burkholderia pyrrocinia–like | 6 ± 1 b, x | 8 ± 1 c, x | −2.69 ± 0.34 b, x | −4.81 ± 0.57 b, y | 3.93 ± 0.24 a, x | 2.02 ± 0.24 a, y | 48 ± 4 ab | 269 ± 32 ab |
No inoculation | 4 ± 1 b, y | 32 ± 3 bc, x | −1.33 ± 0.03 a, x | −2.15 ± 0.09 a, y | 3.80 ± 0.16 a, x | 2.04 ± 0.16 a, y | 45 ± 1 c | 203 ± 13 c |
Mean data (n = 6) of each media treatment (in column) followed by the same letter (a, b, or c) are not significantly different among the inoculation treatments (P < 0.05); Mean data of each inoculation treatment (in line) followed by the same letter (x or y) are not significantly different among the media treatments (P < 0.05). For the plants used for the N2-fixation measurements, foliar δ15N values were −0.61 ± 0.08 and −0.71 ± 0.21 for non-N2-fixing B. polycarpa and Michelia macclurei, respectively. N2-fixation was calculated by Eq. (1) using the averaged foliar δ15N values of these two non-N2-fixing plants.