Figure 4.

Mice lacking Ccrl2 displayed exaggerated neutrophil recruitment during acute inflammation independently of stimulus. 8- to 10-week-old male Ccrl2^−/− mice or age-matched littermate controls were injected with 4% thioglycollate, and 1 h later, animals were sacrificed. Peritoneal cavities were lavaged with ice-cold PBS supplemented with 2 mM EDTA. Cells were quantified using counting beads, and cell populations were analysed using flow cytometry. (A) Representative flow cytometry plots of the peritoneal cavities of wild-type (WT) and Ccrl2^−/− mice treated with thioglycollate. Neutrophils were defined as Ly6B.2 (7/4)^hi and Ly6G^hi (B,C) Total peritoneal cell counts following 1-h thioglycollate challenge. (D) CXCL1 and chemerin (E) levels in the peritoneum of Ccrl2^−/− and WT mice quantified by ELISA. Total blood leucocytes (F) and neutrophils (G) in WT and Ccrl2^−/− mice. (H) Plasma CXCL1 and chemerin (I) levels in Ccrl2^−/− and WT quantified by ELISA. Mean ± SEM. n = 5 animals/group and n = 1 experiment. Statistical significance was assessed using a Student’s unpaired t-test. *P ≤ 0.05.