Figure 7.

Absence of Ccrl2 had no effect on the migratory behaviour of leucocytes towards macrophage or neutrophil chemoattractants. 8- to 10-week-old male Ccrl2^−/− mice and age-matched littermate controls were injected i.p. with 2% Bio-gel (polyacrylamide beads) and sacrificed 4 days later. Peritoneal cavities were lavaged with ice-cold PBS supplemented with 2 mM EDTA. (A) Representative histogram cytometry plots displaying Cmklr1 expression on biogel elicited macrophages and neutrophils. Macrophages were defined as F4/80^hi, Ly6B.2 (7/4)^lo, Ly6G^lo, neutrophils were defined as Ly6B.2 (7/4)^hi, Ly6G^hi. (B,E) A gradient of 5 nM of the indicated chemoattractant was allowed to form, and chemotaxis was measured of 4 × 10⁵ cells (400,000 cells/well) for 3 h. Representative chemotaxis traces of wild type and Ccrl2^−/− peritoneal exudate cell chemotaxis to 5 nM chemerin (B) or 5 nM C5a. (C). (D) Max–Min analysis and (E) slope analysis. Error bars are SEM of four experiments with independent macrophage preparations. Significance was assessed using two-way analysis of variance with Sidak’s multiple comparison test. ns = P > 0.05.