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. 2017 Nov 21;8:2295. doi: 10.3389/fmicb.2017.02295

FIGURE 4.

FIGURE 4

Reduced phospholipid binding capacity (A) and antifungal activity (B) of HsAFP1[H32A][R52A] compared to native HsAFP1. Data are means ± SEM, for n ≥ 3 experiments. (A) Optical density (OD405 nm) values representing the interaction of 12.5 μM HsAFP1 or HsAFP1[H32A][R52A] with all phospholipids tested [phosphatidic acid (PA), phosphatidylinositol(3,5)bisphosphate (PtdIns(3,5)P2), phosphatidylinositol(4,5)bisphosphate (PtdIns(4,5)P2)], determined via rELISA assays. Significant differences between HsAFP1 and HsAFP1[H32A][R52A] were determined via two-way ANOVA followed by Sidak multiple comparison, with ∗∗∗ and ∗∗∗∗ representing P < 0.001 and P < 0.0001, respectively. (B) Minimum Inhibitory Concentration of HsAFP1 or HsAFP1[H32A][R52A] resulting in 50% cell growth reduction (MIC50) of F. culmorum and Minimum Fungicidal Concentration of HsAFP1 or HsAFP1[H32A][R52A] resulting in 50% cell death (MFC50) of S. cerevisiae. Significant differences between HsAFP1 and HsAFP1[H32A][R52A] were determined via an unpaired student t-test with Welch’s correction, with ∗ representing P < 0.05.