FIGURE 5.

Phosphate-dependent binding of HsAFP1 to phosphatidic acid (PA), determined via rELISA assays. Interaction of 12.5 μM HsAFP1 with PA or methyl-PA (A) or for the interaction of HsAFP1 with PA in buffers with different phosphate concentrations (B). Data are means ± SEM, for n ≥ 3 experiments. Data are expressed relative to the HsAFP1-PA binding (A) or relative to the HsAFP1-PA binding in demi-water (0 M NaH₂PO₄) (dotted line) (B). Significant differences between PA and methyl-PA interactions were determined via an unpaired student t-test with Welch’s correction (A), while significant differences between demi-water (0M NaH₂PO₄) and phosphate buffers were determined via one-way ANOVA followed by Dunnett multiple comparison (B), with ^∗∗ and ^∗∗∗ representing P < 0.01 and P < 0.001, respectively.