FIGURE 7.

BODIPY-HsAFP1 is located intracellularly in membrane-permeabilized cells and at the cell surface of non-membrane permeabilized S. cerevisiae cells treated with 16 μM BODIPY-HsAFP1. Confocal microscope images of 60 min treated S. cerevisiae cultures with 16 μM BODIPY-HsAFP1. Propidium iodide (PI; 2 μg/mL) was used to identify membrane permeabilization. (A) Confocal images acquired with normal laser intensities (Alexa fluor 488-10% (argon laser)-HV573 and PI-1% (559 nm laser)-HV600). (B) Confocal images taken at high laser intensities [Alexa fluor 488-10% (argon laser)-HV700 and PI-1% (559 nm laser)-HV600], without impacting autofluorescence. Representative BODIPY-HsAFP1 accumulation spots at the cell surface are indicated with arrows. Bar: 5 μm.