FIGURE 8.

Kinetics of HsAFP1 internalization and membrane permeabilization in S. cerevisiae treated with (A) low (4 μM) or (B) high (48 μM) BODIPY-HsAFP1 (B-Hs) and 2 μg/mL propidium iodide (PI), determined via flow cytometry. The gray dashed line represents the percentage of B-Hs+ yeast cells in the population, relative to control (MQ water) treatment, which can be further divided in two subpopulations: yeast cells with (PI+; orange squares) and without (PI–; black circles) compromised membranes. Data are means ± SEM, for n = 3 experiments. To analyze significant differences in the size of the subpopulations between the time zero and other time points, one-way ANOVA followed by Dunnett multiple comparison was performed.