Skip to main content
. 2017 Nov 21;7:479. doi: 10.3389/fcimb.2017.00479

Figure 7.

Figure 7

Gene expression changes post virus infection and transfection of circRNA-chr19-targeting microRNAs. (A) CircRNA-chr19 expression in 293T cells was analyzed via qPCR at 24 h and 0 h post virus infection and compared. (B) The expression levels of circRNA-chr19 and CLDN18 in 293T cells infected by virus. RNA expression, which was normalized by the expression level of GAPDH, is represented by the 2−ΔΔt value. (C) MiR-30b-3p matches the 3′-UTRs of CLDN18 and circRNA-chr19 (indicated by solid lines). The interactions of circRNAs and mRNAs with miRNAs were predicted by miRNA target prediction software, such as TargetScan and Bielefeld Bioinformatics Service online resource. (D) Wild-type and mutant CLDN18 3′-UTR activity were analyzed by luciferase reporter assays. The vector expressing miR-30b-3p was co-transfected with the vector containing the wild-type or mutant 3′-UTR. The results are presented as the mean ± SD of 3 different preparations, *P < 0.05.