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. 2017 Nov 21;8:2281. doi: 10.3389/fmicb.2017.02281

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Copyright © 2017 Nácher-Vázquez, Ruiz-Masó, Mohedano, del Solar, Aznar and López.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genetical map of plasmid pMN1 (A) and determination of its copy number (B,C). (A) The genes and orf of pMN1 are depicted. (B) Results of the pMN1 copy-number determination are summarized in this table. (C) For each gene, determined mean Ct values were plotted against the logarithm of the four amounts of template DNA used in the qPCR assays. PCR amplification efficiencies (E values) were calculated from the slope of the curves generated by linear regression through the experimental points.