Table 1.
Description of bacteria used in this work.
| Strain | Plasmids | aResistance | Information | References |
|---|---|---|---|---|
| Lb. sakei MN1 (CECT 8329) | pMN1 pMN2 |
– | Isolated from a fermented meat product | Chenoll et al., 2007 |
| Lb. sakei MN1[pRCR12] | pMN1 pMN2 pRCR12 |
CmR | Derivative of pRCR. It contains the promoter Px of malXCD operon of Streptococcus pneumoniae JNR7/87 fused to the mrfp gene | Nácher-Vázquez et al., 2017 |
| Lb. sakei MN1[pRCR13] | pMN1 pMN2 pRCR13 |
CmR | Derivative of pRCR by cloning of region A (GenBank MF590088 position: 38–363) upstream of mrfp | This study |
| Lb. sakei MN1[pRCR14] | pMN1 pMN2 pRCR14 |
CmR | Derivative of pRCR by cloning of region B (GenBank MF590088 position: 1,476–1,819) upstream of mrfp | This study |
| Lb. sakei MN1[pRCR15] | pMN1 pMN2 pRCR15 |
CmR | Derivative of pRCR by cloning of region C (GenBank MF590088 position: 2,392–2,749) upstream of mrfp | This study |
| L. lactis MG1363 | – | – | Strain derived from L. lactis 712 by plasmids curing | Gasson, 1983 |
| L. lactis MG1363[pRCR13] | pRCR13 | CmR | Derivative of pRCR by cloning of region A upstream of mrfp | This study |
| L. lactis MG1363[pRCR14] | pRCR14 | CmR | Derivative of pRCR by cloning of region B upstream of mrfp | This study |
| L. lactis MG1363[pRCR15] | pRCR15 | CmR | Derivative of pRCR by cloning of region C upstream of mrfp | This study |
| E. coli DH5α[pRCR] | pRCR | CmR | Promoters probe vector containing the mrfp gene, which encodes the fluorescent mCherry protein | Mohedano et al., 2015 |
| E. coli V517 | Eight plasmids pVA517A through pVA517H | ND | Source of plasmids used as references in agarose gel analysis | Macrina et al., 1978 |
a
ND, No determined; CmR, resistance to chloramphenicol.