Table 2.
Description of primers used in this work.
| Target | Primera | Sequence (5′-3′) | Product (bp) |
|---|---|---|---|
| pcrA | pcrF | ACAAACATGGCGCATCAACG | 146 |
| pcrR | GCGAAGGTGCTCAAGATGTTT | ||
| repA | repF | GGCAAGCCGGTTATTGGTTAC | 140 |
| repR | TTTTCCTGCTCTGTTAATTCACCAT | ||
| dsrLS | dsrF | GATGATGGTCAATATATGGCAA | 695 |
| dsrR | CTTGAACGATATTGTGGTGCCAA | ||
| Region A | P1F | GAAGATCTTCTTTTAGACCCCTCTTGAGGCT | 343 |
| P1R | GCTCTAGAGCAGTATCATCACCTTTATCGCGC | ||
| Region B | P2F | GGAAGATCTTCCTCAGCAACAACGGTTAGCCT | 366 |
| P2R | GCTCTAGAGCCGCCAGTGATCATATAACCGA | ||
| Region C | P3F | GGAAGATCTTCCAAATTAACCAGAGACCGC | 376 |
| P3R | GCTCTAGAGCTGGCTGGCTGGTAACTAGCA | ||
| RT-PCR Amplicon 1 | rt1F | AGCTGGGTTCGATATGCTTTA | 1,150 |
| rt1R | CCCACCCCTCGCTCTTTA | ||
| RT-PCR Amplicon 2 | P2F | GGAAGATCTTCCTCAGCAACAACGGTTAGCCT | 1,952 |
| rt2R | CGGTTGGCAAAGACGTTTTG | ||
| RT-PCR Amplicon 3 | P3F | GGAAGATCTTCCAAATTAACCAGAGACCGC | 1,033 |
| rt2R | CGGTTGGCAAAGACGTTTTG | ||
| RT-PCR Amplicon 4 | rt2F | ACGGCTGCGATCACTACTG | 496 |
| rt2R | CGGTTGGCAAAGACGTTTTG | ||
| RT-PCR Amplicon 5 | rt3F | AGCTTACGCTGCTACCAAGGC | 862 |
| rt3R | ATGGCTGGAGTAAAATGGATCAGCT |
a
F, Forward; R, reverse.