Table 4.
Fluorescent detection of promoter regions in Lb. sakei by expression of translational fusions to the mrfp gene.
| Growth phase | aMiddle exponential phase | aLate stationary phase | ||
|---|---|---|---|---|
 |
Glucose | Sucrose | Glucose | Sucrose |
| Lb. sakei MN1 | 1.3 ± 0.6 | 0.9 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.5 |
| Lb. sakei MN1[pRCR12] | 355 ± 30 | 351 ± 18 | 227 ± 14 | 139 ± 5.5 |
| Lb. sakei MN1[pRCR13] | 142 ± 14 | 76 ± 7.5 | 149 ± 11 | 68 ± 2.2 |
| Lb. sakei MN1[pRCR14] | 4.6 ± 0.4 | 2.0 ± 0.2 | 5.5 ± 0.3 | 1.6 ± 0.1 |
| Lb. sakei MN1[pRCR15] | 73 ± 2.6 | 28 ± 2.0 | 40 ± 0.9 | 8.7 ± 0.4 |
a
The specific fluorescence is depicted and it was calculated as the ratio of the detected fluorescence (10 ×) and the bacterial biomass estimated from the A600 of the culture.