Table 1.
Strains and plasmids used.
| Strains/Plasmids | Relevant genotype or description | Source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk−, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA | Invitrogen Corp. |
| S17-1 | λpir; hsdR pro recA; RP4 2-Tc::Mu-Km::Tn7, pro, res−, mod+, StrR, TrmR | (Simon et al., 1983) |
| Pseudomonas aeruginosa | ||
| PAO1 | Wild type strain PAO1 | B.H. Holloway |
| ΔsagS | PAO1, ΔsagS (PA2824) | (Petrova and Sauer, 2011) |
| ΔsagSΔgacA | ΔsagS, ΔgacA in PAO1 | (Petrova and Sauer, 2011) |
| ΔrsmYZ | ΔrsmYΔrsmZ in PAO1 | (Kay et al., 2006) |
| ΔrsmA | ΔrsmA in PAO1 | (Kay et al., 2006) |
| ΔhptB | ΔhptB in PAO1; KmR | (Lin et al., 2006) |
| ΔcafA | ΔcafA in PAO1 | (Petrova and Sauer, 2010) |
| Plasmids | ||
| pCR2.1-TOPO | TA cloning vector; KmR; AmpR | Invitrogen Corp. |
| pRK2013 | Helper plasmid for triparental mating; mob; tra; KmR | (Figurski and Helinski, 1979) |
| pJN105 | Arabinose-inducible gene expression vector; pBRR-1 MCS; araC-PBAD; GmR | (Newman and Fuqua, 1999) |
| pMJT1 | araC-PBAD cassette of pJN105 cloned into pUCP18, AmpR (CarbR) | (Kaneko et al., 2007) |
| pminiCTX-lacZ | attB site-specific integration vector, TetR | (Becher and Schweizer, 2000) |
| pJN-sagS | C-terminal HA-tagged sagS cloned into pJN105 at NheI/SacI; GmR | (Petrova and Sauer, 2011) |
| pJN-sagS_H315A | C-terminal HA-tagged sagS with H315 mutation in pJN105 at NheI/SacI; GmR | This study |
| pJN-sagS_H348A | C-terminal HA-tagged sagS with H348A mutation in pJN105 at NheI/SacI; GmR | This study |
| pJN-sagS_D713A | C-terminal HA-tagged sagS with D713A mutation in pJN105 at NheI/SacI; GmR | This study |
| pJN-sagS-HmsP-HisKA | C-terminal HA-tagged SagS HmsP-HisKA domains cloned into pJN105 at NheI/SacI; GmR | This study |
| pJN-sagS-HisKA | C-terminal HA-tagged SagS HisKA domain cloned into pJN105 at NheI/SacI; GmR | This study |
| pJN-sagS-HisKA-Rec | C-terminal HA-tagged SagS HisKA-Rec domain cloned into pJN105 at NheI/SacI; GmR | This study |
| pJN-sagS-Rec | C-terminal HA-tagged SagS Rec domain cloned into pJN105 at NheI/SacI; GmR | This study |
| pJN-rsmY | rsmY cloned into pJN105 at EcoRI/SacI; GmR | (Petrova and Sauer, 2010) |
| pJN-rsmZ | rsmZ cloned into pJN105 at EcoRI/SacI; GmR | (Petrova and Sauer, 2010) |
| pMJT-bfiS | C-terminal His6/V5-tagged bfiS cloned into pMJT1; AmpR (CarbR) | (Petrova and Sauer, 2011) |
| pMJT-brlR | C-terminal His6/V5-tagged brlR cloned into pMJT1; AmpR (CarbR) | (Liao and Sauer, 2012) |
| pMJT-sagS | C-terminal HA-tagged sagS cloned into pMJT1 at NheI/SacI; GmR | (Petrova and Sauer, 2011) |
| pMJT-sagS-HmsP-HisKA | C-terminal HA-tagged SagS HmsP-HisKA domains cloned into pMJT1 at NheI/SacI; AmpR (CarbR) | This study |
| pMJT-sagS-HmsP-HisKA_H315A | C-terminal HA-tagged SagS HmsP-HisKA domains with H315A mutation cloned into pMJT1; AmpR (CarbR) | This study |
| pMJT-sagS-HisKA | C-terminal HA-tagged SagS HisKA domain cloned into pMJT1; AmpR (CarbR) | This study |
| pMJT-sagS-HisKA-Rec | C-terminal HA-tagged SagS HisKA-Rec domain cloned into pMJT1; AmpR (CarbR) | This study |
| pMJT-sagS-Rec | C-terminal HA-tagged SagS Rec domain cloned into pMJT1; AmpR (CarbR) | This study |
| pMJT-sagS-Rec_D713A | C-terminal HA-tagged SagS Rec domain with D713A mutation cloned into pMJT1 at NheI/SacI; GmR | This study |
| pMJT-sagS-Rec_D713E | C-terminal HA-tagged SagS Rec domain with D713E mutation cloned into pMJT1 at NheI/SacI; GmR | This study |
| pCTX-rsmY-lacZ | rsmY reporter construct in mini-CTX-lacZ; TetR | (Brencic and Lory, 2009) |
| pCTX-rsmZ-lacZ | rsmZ reporter construct in mini-CTX-lacZ; TetR | (Brencic and Lory, 2009) |
| pCTX-PbrlR-lacZ | brlR promoter reporter construct in mini-CTX-lacZ, using PbrlR-lacZF/R primers; TetR | (Liao and Sauer, 2012) |
| PbrlR-brlR-His6V5 | pMini-CTX harboring C-terminal His6/V5-tagged brlR under the control of the brlR promoter region (1–120 bp upstream of the brlR start codon) | (Gupta et al., 2014) |