. Author manuscript; available in PMC: 2017 Nov 26.

Published in final edited form as: Environ Microbiol. 2017 Apr 3;19(5):2005–2024. doi: 10.1111/1462-2920.13719

Table 2.

Primers used in this study.

Oligonucleotide	Sequence^a,^b
	*Inducible Expression Constructs*
HmsP-HisKA-for	GCGCGCGCgctagcATGCTAGGCGGCAGAACCTCGC
HmsP-HisKA-HA-rev	GCGCGCGCgagctcCTAagcgtagtctgggacgtcgtatgggtaGCGCTGTTCTCCCGTGGTG
HisKA–for	GCGCGCGCgctagcATGCTCGGGCGCATCTCGGTAG
HisKA–HA-rev	GCGCGCGCgagctcCTAagcgtagtctgggacgtcgtatgggtaCAGGACCCGGGTGTTGCT
HisKA-Rec-for	GCGCGCGCgctagcATGCTCGGGCGCATCTCGGTAG
HisKA-Rec-HA-rev	GCGCGCGCgagctcCTAagcgtagtctgggacgtcgtatgggtaGTCGCTCGCGGTGAGCGG
Rec-for	GCGCGCgctagcATGGTCAGCCCTCCGCTGCAG
Rec-HA-rev	GCGCGCGCgagctcCTAagcgtagtctgggacgtcgtatgggtaGTCGCTCGCGGTGAGCGG
	*Site-directed Mutagenesis*
sagS_H315A_for	CTTCCTGGCCAACATGAGCGCCGAGATCCGCACGCCGCTG
sagS_H315A_rev	CAGCGGCGTGCGGATCTCGGCGCTCATGTTGGCCAGGAAG
sagS_H348_for	GCCAGCAACTGTCGATCGCCGCCGACTCCGGCAAGGTGCTGGTG
sagS_H348_rev	CACCAGCACCTTGCCGGAGTCGGCGGCGATCGACAGTTGCTGGC
sagS_D713A_for	CCATCGACCTGGTCCTGATGGCCTGCAACATGCCAGTGATGGAC
sagS_D713A_rev	GTCCATCACTGGCATGTTGCAGGCCATCAGGACCAGGTCGATGG
sagS_D713E_for	CCATCGACCTGGTCCTGATGGAGTGCAACATGCCAGTGATGGAC
sagS_D713E_rev	GTCCATCACTGGCATGTTGCACTCCATCAGGACCAGGTCGATGG
	*Sequencing*
sagScheck_rev	GCAGTACCTGGTCATGCTGG
sagScheck_for	CGATCTCGATCAGGCGCAG
	qRT-PCR
mreB-for	CTGTCGATCGACCTGGG
mreB-rev	CAGCCATCGGCTCTTCG
RsmZ-for	GAAGGATCGGGGAAGGGAC
RsmZ-rev	CGCCCACTCTTCAGTCCC

, Restriction sites are indicated by nucleotides in lower case

, HA tag codons are underlined and indicated in lower case letters