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. 2017 Nov 21;8:924. doi: 10.3389/fphys.2017.00924

Figure 2.

Figure 2

Immunostaining of NKCC1 protein in HAT-7 cells, developing mouse dental epithelium and mouse salivary glands. NKCC1 expression as green punctuate grains near the plasma membranes of cultured HAT-7 cells stained with anti-NKCC1 (A) or with non-immune IgG (B, control) and visualized using Alexa488-coupled secondary antibody. Panels (C,D) show salivary glands of a wild type (C) and Nkcc1 null mouse (G) stained with anti-NKCC1. NKCC1 staining in mouse dental epithelium at secretory stage (F) and in maturation stage (E,G) in papillary layer. Absence of staining with anti-NKCC1 in salivary gland (D) and incisor (H) of a Nkcc1-null mouse confirms the specificity of primary antibody. Note: all the stainings have been tested in triplicate in three mice. E, enamel; ES, enamel space; SA, ameloblasts secretory stage; MA, ameloblasts maturation stage; P, pulp; PL, papillary layer; SI, stratum intermedium.