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. 2017 Nov 21;8:924. doi: 10.3389/fphys.2017.00924

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Copyright © 2017 Jalali, Lodder, Zandieh-Doulabi, Micha, Melvin, Catalan, Mansvelder, DenBesten and Bronckers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative tracings showing the effect of 10 μM bumetanide on the channel activity in HAT-7 cells. Left figure shows the channel activity obtained from cell-attached patch with aCSF in the recording pipet at 0 mV. Right figure shows the channel activity obtained when the recording pipet was filled with aCSF + 10 μM bumetanide and clamped at 0 mV.