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. Author manuscript; available in PMC: 2017 Nov 26.
Published in final edited form as: Methods Enzymol. 2015 Apr 27;560:187–217. doi: 10.1016/bs.mie.2015.03.009
5 µL 10× Pfu DNA polymerase buffer
2 µL 10 mM dNTPs
2 µL 10 ng/µL pTRM4-WT plasmid
2 µL 10 µM TRM4-TAA-F1 primer
2 µL 10 µM TRM4-TAA-R1 primer
1 µL 5U/µL Pfu DNA polymerase
Add ddH2O to a final reaction volume of 50 µL.