5 µL | 10× Pfu DNA polymerase buffer |
2 µL | 10 mM dNTPs |
2 µL | 10 ng/µL pTRM4-WT plasmid |
2 µL | 10 µM TRM4-TAA-F1 primer |
2 µL | 10 µM TRM4-TAA-R1 primer |
1 µL | 5U/µL Pfu DNA polymerase |
Add ddH2O to a final reaction volume of 50 µL. |
5 µL | 10× Pfu DNA polymerase buffer |
2 µL | 10 mM dNTPs |
2 µL | 10 ng/µL pTRM4-WT plasmid |
2 µL | 10 µM TRM4-TAA-F1 primer |
2 µL | 10 µM TRM4-TAA-R1 primer |
1 µL | 5U/µL Pfu DNA polymerase |
Add ddH2O to a final reaction volume of 50 µL. |