10 µL | 1 µg/µL BamHI-digested PCR product (snR81-TRM4-TAA or snR81-Control) |
5 µL | 10× HindIII digestion buffer |
2.5 µL | HindIII (10 U/µL) |
Add ddH2O to a final reaction volume of 50 µL. |
10 µL | 1 µg/µL BamHI-digested PCR product (snR81-TRM4-TAA or snR81-Control) |
5 µL | 10× HindIII digestion buffer |
2.5 µL | HindIII (10 U/µL) |
Add ddH2O to a final reaction volume of 50 µL. |