5 µL | Digested PCR product (snR81-TRM4-TAA or snR81-Control) |
1 µL | Digested pSEC plasmid |
2 µL | 5× T4 DNA ligase buffer |
2 µL | T4 DNA ligase (1 U/µL) |
5 µL | Digested PCR product (snR81-TRM4-TAA or snR81-Control) |
1 µL | Digested pSEC plasmid |
2 µL | 5× T4 DNA ligase buffer |
2 µL | T4 DNA ligase (1 U/µL) |