Skip to main content
. Author manuscript; available in PMC: 2017 Nov 26.
Published in final edited form as: Cell Tissue Res. 2009 Apr 2;336(2):213–223. doi: 10.1007/s00441-009-0776-z

Fig. 5.

Fig. 5

TGFβ stimulates the phosphorylation of mTOR, p70S6K, and 4E-BP1 in cells treated for variable lengths of time before lysis. a Top Typical Western blot probed for phosphorylated mTOR, stripped, and reprobed for total mTOR and GAPDH. Bottom Densitometric results as the ratio of phosporylated to total mTOR normalized to GAPDH (n=10, *P<0.05, #P<0.001). Control phosphorylation was defined at 1.0. b Top Typical Western blot probed for phosphorylated 4E-BP1, stripped, and reprobed for total 4E-BP1 and GAPDH. Bottom Densitometric results as the ratio of phosphorylated to total 4E-BP1 normalized to GAPDH (n=10, *P<0.04). Control phosphorylation was defined at 1.0. c Top Typical Western blot probed for phosphorylated p70S6K, stripped, and reprobed for total p70S6K and GAPDH. Bottom Densitometric results as the ratio of phosporylated to total p70S6K normalized to GAPDH (n=10, *P<0.02). Control phosphorylation was defined at 1.0