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. Author manuscript; available in PMC: 2017 Nov 26.
Published in final edited form as: Cell Tissue Res. 2009 Apr 2;336(2):213–223. doi: 10.1007/s00441-009-0776-z

Fig. 6.

Fig. 6

TGFβ stimulated mTOR, p70S6K, and 4E-BP1 phosphorylation in cells treated for 5 min is abolished by pre-treatment with the MEK inhibitor PD98059 (10 μM) for 1 h. a Typical Western blot probed for phosphorylated mTOR, phosphorylated p70S6K, phosphorylated 4E-BP1, and GAPDH for the indicated times. b–d Densitometric results as the ratio of phosporylated protein normalized to GAPDH (n≥4, *P<0.05). Control phosphorylation (Ctr) at time point 0 was defined at 1.0