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. 2017 Oct 31;6(12):2146–2159. doi: 10.1002/sctm.17-0137

Figure 5.

Figure 5

P21‐RUNX2‐8R significantly inhibits chondrogenic genes under osteogenic conditions. Human mesenchymal stem cells (hMSCs) were cultured in osteo‐inductive media (with 100 nM Dexamethasone) with or without protein transduction under two different seeding conditions mentioned below. The cells were washed and total RNA was extracted after 2 weeks. After DNAse treatment, gene expression analysis of osteogenic genes (RUNX2 and OPN) and chondrogenic genes (Aggrecan and SOX9) of P21‐RUNX2‐8R transduced and nontransduced hMSCs was performed. The cells were cultured as a high‐density monolayer on flat bottom plate (A) or as aggregate masses (B) on a round bottom plate in osteo‐inductive medium with 10 nM Dexamethasone (OST) or chondrogenic (CHON) medium. The results were portrayed in a graph based on expression fold change to un‐transduced hMSCs cultured in basal media (dotted line). Statistical analysis was performed using two‐way ANOVA, with α = 0.05; *, p ≤ .05. Error bars indicate SD. hMSCs from two different donors were used for this study. Abbreviation: CHON, chondrogenic; OPN, Osteopontin.