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. 2017 Oct 24;6(12):2126–2134. doi: 10.1002/sctm.17-0033

Figure 3.

Figure 3

SEM analysis and Fluorescence microscopy images. (A1–A3, B1–B3): Human dental pulp stem cells (hDPSCs) from each group were attached and spread well on the calcium phosphate cement (CPC) scaffolds on day 1. (C1–C3, D1–D3): By day 3, hDPSCs from each group had grown well and the cells elongated to find each other to form cellular connections. (E1, F1): Fluorescence microscopy images of Lenti‐LacZ‐transfected hDPSCs and Lenti‐PDGF‐BB transfected hDPSCs on CPC scaffold after culture 12 hours in vitro. (E2–E3, F2–F3): 3D reconstructed Laser confocal scanning microscopy images of Lenti‐LacZ‐transfected hDPSCs and Lenti‐PDGF‐BB transfected hDPSCs on CPC scaffold after culture 3 days in vitro. Cell nuclei were stained with DAPI. Scale bar, 100 μm. Abbreviations: PDGF, platelet‐derived growth factor; DAPI, 4′,6‐diamidino‐2‐phenylindole.