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. 2017 Oct 19;6(12):2160–2172. doi: 10.1002/sctm.17-0133

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© 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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In vitro osteogenic differentiation of BMSC and ATSC when MSC were cultured with or without standard osteogenic induction media (250 μM ascorbic acid, 10 mM β‐glycerolphosphate, and 100 nM dexamethasone) for up to 21 days. BMSC of three different human donors (n = 3) and ATSC of four different human donors (n = 4) were plated in triplicate for each time point. (A): Extracellular ALP secretion is shown qualitatively by Fast Blue RR salt and Naphthol AS‐MX phosphates alkaline staining. (B): Intracellular ALP activity, data are presented as mean ± SD. (C): Mineralization was qualitatively assessed by alizarin red (AR) staining and representative images from each group are presented. (D): AR staining was solubilized and enumerated per well and normalized to DNA content, presented as mean ± SD. * indicates statistical differences (p < .05) between groups. Abbreviations: ALP, alkaline phosphatase; ATSC, adipose tissue‐derived stem cells; BMSC, bone marrow‐derived stem cells.